Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Microbial degradation of alkyl carbazoles in Norman wells crude oil.

Norman Wells crude oil was fractionated by sequential alumina and silicic acid column chromatography methods. The resulting nitrogen-rich fraction was analyzed by gas chromatography-mass spectrometry and showed 26 alkyl (C(1) to C(5)) carbazoles to be the predominant compounds. An oil-degrading mixed bacterial culture was enriched on carbazole to enhance its ability to degrade nitrogen heterocy...

Microbial utilization of crude oil.

The utilization of two crude oil samples of different quality at 4 and 30 C has been studied by using pure and mixed bacterial cultures obtained by enrichment procedures. Growth, emulsification, and utilization occurred readily at both temperatures. The crude oil residue is increased in specific gravity and readily sediments out of solution. A comparison of the chemical analysis of the oils by ...

Indigenous production of biosurfactant and degradation of crude oil

The present study investigated the isolation and identification of biosurfactant producing bacteria from Iranian oil wells. The biosurfactant production of bacteria isolates was evaluated and confirmed using hemolysis and emulsification tests. The biodegradation of crude oil was studied using GC and HPLC analysis. A total of 45 strains have been isolated. These strains showed less than a 40 mN ...

Biosurfactant and Degradative Enzymes Mediated Crude Oil Degradation by Bacterium Bacillus subtilis A1

In this work, the biodegradation of the crude oil by the potential biosurfactant producing Bacillus subtilis A1 was investigated. The isolate had the ability to synthesize degradative enzymes such as alkane hydroxylase and alcohol dehydrogenase at the time of biodegradation of hydrocarbon. The biosurfactant producing conditions were optimized as pH 7.0, temperature 40°C, 2% sucrose and 3% of ye...

Degradation of Crude Oil by Selected Marine Yeasts